MSC to adipocyte (mouse): Difference between revisions
From FANTOM5_SSTAR
No edit summary |
No edit summary |
||
Line 29: | Line 29: | ||
''Osteoblast differentiation:''<br> | ''Osteoblast differentiation:''<br> | ||
Osteogenic differentiation was induced by changing the medium every three days to culture medium supplemented with 100 ng/ml of bone morphogenetic protein 4 (BMP4, R&D Systems, Mineapolis, MN) [3]. Pre-conditioned medium which was originally same as culure medium, was carried by parallel culturing of ST2, to avoid the perturbation by "medium-change" shock as soon as possible.<br> | Osteogenic differentiation was induced by changing the medium every three days to culture medium supplemented with 100 ng/ml of bone morphogenetic protein 4 (BMP4, R&D Systems, Mineapolis, MN) [3]. Pre-conditioned medium which was originally same as culure medium, was carried by parallel culturing of ST2, to avoid the perturbation by "medium-change" shock as soon as possible.<br> | ||
<html><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/ALP6d.png'></html><br> | <html><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/ALP20d.png'><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/ALP6d.png'><img src='https://fantom5-collaboration.gsc.riken.jp/resource_browser/images/TC_qc/ALP0d.png'></html><br> | ||
Figure 2. ALP staining of ST2 cells for 0, 6 and 20 days after osteoblast induction.<br> | Figure 2. ALP staining of ST2 cells for 0, 6 and 20 days after osteoblast induction.<br> | ||
The ALP activity of ST2 cells (right, 20 days) were more prominently increased in the presence of BMP4 than 0 day (left).<br> | The ALP activity of ST2 cells (right, 20 days) were more prominently increased in the presence of BMP4 than 0 day (left).<br> |
Revision as of 16:27, 11 December 2014
Series: | IN_VITRO DIFFERENTIATION SERIES |
---|---|
Species: | Mouse (Mus musculus) |
Genomic View: | Zenbu |
Expression table: | [{{{tet_config}}} FILE] |
Link to TET: | [{{{tet_file}}} TET] |
Sample providers : | Yasushi Okazaki |
Germ layer: | {{{germ_layer}}} |
Primary cells or cell line: | {{{primary_cells}}} |
Time span: | {{{time_span}}} |
Number of time points: | {{{number_time_points}}} |
CollapseOverview |
---|
Senile osteoporosis is the most common metabolic bone disease. This disease is often accompanied by increasing adipocytes in bone marrow tissues [1]. The ectopic adipocytes differentiation following bone loss seems to be caused by unbalanced differentiation of mesenchymal stem cells (MSCs) [2]. Although several differentiation regulators of MSCs have already been reported, little is known about the regulatory dynamics of bi-directional adipocytes/osteoblasts differentiation. |
ExpandSample description |
---|
ExpandQuality control |
---|
Profiled time course samples
Only samples that passed quality controls (Arner et al. 2015) are shown here. The entire set of samples are downloadable from FANTOM5 human / mouse samples