Protocols:RNA extraction: Difference between revisions
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We have used various RNA extraction protocols in FANTOM project. | We have used various RNA extraction protocols in FANTOM project. | ||
All the following, but RNA extraction by trizol and ethanol, which was modified in order to keep both small and long RNA in the prep step, are standard. | |||
*OP-RNA-extraction-totalRNA-TRIzol-ethanol-v1.0 | *OP-RNA-extraction-totalRNA-TRIzol-ethanol-v1.0 | ||
** [[File:Trizol_ethanol_ver1.pdf]] | |||
*OP-RNA-extraction-totalRNA-TRIzol-isopropanol-v1.0 | *OP-RNA-extraction-totalRNA-TRIzol-isopropanol-v1.0 | ||
** [[File:Trizol_isopropanol_ver1.pdf]] | |||
*OP-RNA-extraction-totalRNA-miRNeasy_Mini-v1.0 | *OP-RNA-extraction-totalRNA-miRNeasy_Mini-v1.0 | ||
** [[File:MiRNEasy_ver1.pdf]] | |||
*OP-RNA-extraction-totalRNA-RiboPure_minelute_cleanup-v1.0 | *OP-RNA-extraction-totalRNA-RiboPure_minelute_cleanup-v1.0 | ||
** [[File:RNeasy_MinElute_Cleanup_Handbook.pdf]] | |||
** [[File: Cms_059303.pdf]] | |||
*OP-RNA-extraction-totalRNA-ToTALLY-RNA-v1.0 | *OP-RNA-extraction-totalRNA-ToTALLY-RNA-v1.0 | ||
** [[File:Totally_RNA_ver1.pdf]] | |||
Latest revision as of 13:35, 3 September 2012
We have used various RNA extraction protocols in FANTOM project.
All the following, but RNA extraction by trizol and ethanol, which was modified in order to keep both small and long RNA in the prep step, are standard.
- OP-RNA-extraction-totalRNA-TRIzol-ethanol-v1.0
- OP-RNA-extraction-totalRNA-TRIzol-isopropanol-v1.0
- OP-RNA-extraction-totalRNA-miRNeasy_Mini-v1.0
- OP-RNA-extraction-totalRNA-RiboPure_minelute_cleanup-v1.0
- OP-RNA-extraction-totalRNA-ToTALLY-RNA-v1.0
