FFCP PHASE1:Mm9::chr8:41597123..41597141,+: Difference between revisions
From FANTOM5_SSTAR
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{{FFCP | {{FFCP|EntrezGene=52348|HGNC=|MCL_coexpression_id=0|UniProt=Q8CHS8,E9Q7V5,E9Q256,Q3UV99|association_with_transcript=0bp_to_ENSMUST00000098817,ENSMUST00000166612,ENSMUST00000172147,NM_033560,uc009lmv.1,uc009lmw.1,uc009lmx.1,uc009lmy.1_5end|description=CAGE_peak_2_at_Vps37a_5end|id=chr8:41597123..41597141,+|ontology_enrichment_celltype=CL:0000055!4.51e-10!50;CL:0000393!4.24e-09!39;CL:0000211!4.24e-09!39;CL:0000117!1.84e-08!23;CL:0000337!1.84e-08!23;CL:0000221!2.07e-08!44;CL:0000133!2.07e-08!44;CL:0002319!2.13e-08!43;CL:0000540!6.46e-08!33;CL:0000047!6.46e-08!33;CL:0000031!6.46e-08!33;CL:0000404!6.46e-08!33|ontology_enrichment_celltype_v019_2=CL:0000393,3.56e-09,39;CL:0000211,3.56e-09,39;CL:0000117,1.66e-08,23;CL:0000337,1.66e-08,23;CL:0000221,1.76e-08,44;CL:0000133,1.76e-08,44;CL:0002319,1.82e-08,43;CL:0000540,5.82e-08,33;CL:0000047,5.82e-08,33;CL:0000031,5.82e-08,33;CL:0000404,5.82e-08,33|ontology_enrichment_disease=|ontology_enrichment_disease_v019_2=|ontology_enrichment_uberon=UBERON:0000073!3.51e-13!75;UBERON:0001016!3.51e-13!75;UBERON:0001017!3.53e-12!73;UBERON:0005743!3.53e-12!73;UBERON:0004121!1.73e-10!102;UBERON:0000924!1.73e-10!102;UBERON:0006601!1.73e-10!102;UBERON:0001049!1.46e-09!52;UBERON:0005068!1.46e-09!52;UBERON:0006241!1.46e-09!52;UBERON:0007135!1.46e-09!52;UBERON:0002020!7.52e-09!34;UBERON:0000955!1.23e-08!50;UBERON:0006238!1.23e-08!50;UBERON:0002346!1.86e-08!71;UBERON:0003075!1.86e-08!71;UBERON:0007284!1.86e-08!71;UBERON:0002616!3.79e-08!49;UBERON:0000033!7.00e-08!63|ontology_enrichment_uberon_v019_2=UBERON:0001016,3.67e-13,75;UBERON:0001017,3.83e-12,73;UBERON:0004121,6.37e-11,95;UBERON:0000924,6.37e-11,95;UBERON:0006601,6.37e-11,95;UBERON:0000073,1.45e-10,54;UBERON:0010314,7.27e-10,92;UBERON:0001049,1.82e-09,52;UBERON:0005068,1.82e-09,52;UBERON:0006241,1.82e-09,52;UBERON:0007135,1.82e-09,52;UBERON:0002346,3.44e-09,64;UBERON:0003075,3.44e-09,64;UBERON:0007284,3.44e-09,64;UBERON:0002020,6.62e-09,34;UBERON:0010371,4.98e-08,73;UBERON:0000955,4.51e-07,47;UBERON:0006238,4.51e-07,47|phase1_expression=1.28119077683835,1.04692622291808,0.909998478779072,3.93203474698879,4.50806156951712,1.17515490338323,1.16806320297763,2.7115897922731,0.281577444303333,1.63845168425395,1.71217968225505,1.67521533890206,1.54080656938085,2.27293046289393,0.999329285446988,0.404950534779652,1.39912375625629,0.946386496074713,1.7824876368746,0.84877930359606,7.22867966284093,0.595745707205312,0.783599367416806,0.639969691173627,1.37645568640302,1.14846392823004,1.29228598847722,1.939241094173,0.190023419456374,0.252030129759931,0.665191531965914,0.635030673459631,0,0,0.866554859253824,0.332469290716985,0.408338712524738,2.51118978442145,0,1.57057123192139,4.70585190424811,2.04913645487534,1.45653105909346,1.76255545165808,2.33166941804883,2.20256102594018,1.56478268869608,1.96222640742402,1.31811709640683,1.36100929351998,1.67983705950435,1.43752564364972,0.838913030288682,1.45072428402269,0.336093799142464,1.54355661621568,2.29226751951005,4.12537673821954,2.42779753031517,1.5869729378161,1.90149421051854,2.03306719241479,0,0.899899717281399,0.988866362566974,0.7104801149461,1.19840044207362,2.30530983192933,3.52544681848824,2.70011876663381,3.57552434297349,3.42781181284564,4.19650227808066,1.95985278743926,3.73797336971122,0.919218786610021,4.40463523997555,4.97980618532232,5.9906547969935,1.58263545276233,2.74462930807229,0.52091337055676,5.30447698102624,5.06486349028512,5.04234217663473,5.00868595305235,5.69486723196412,6.15118171223492,4.69502398665741,3.47629176491213,1.15945795480671,3.44169550792555,0,0.539703191637817,0.649776417447669,0.896053774194671,3.37066608316204,1.02258193546835,1.03914401841148,1.7666978725517,1.69012103732017,1.28326477414318,2.54805239706646,0,1.94841936910093,1.01343946190039,1.01104889002957,1.94112595522402,2.33044095282455,3.41416919849097,1.56136794089522,0,0.691846491768175,0.753613202763113,1.76383060703557,2.13633205260448,0.988779870937496,1.01219549843938,0,2.10296904931632,1.76673214768315,0.969750506270444,0,2.04946557908083,2.93225389538837,1.02182822083437,0.239630249011841,0,0.508280908292259,4.6435098215495,1.83000241127451,2.51308704225729,2.43354048269187,3.83573185995496,3.53239675751252,3.56930778749067,1.73126918545538,1.24406970610093,2.52722758433675,2.31486041475121,1.17108398666433,1.37941262660084,1.26043490863744,2.10986744970001,1.42876648217788,0.896796041993948,1.87403449119458,1.81092359517311,0.646703978798527,0.189106080167819,1.05570674498369,1.31517439456545,0.834141747676794,1.39300866608867,0.61734640922383,2.38841120182192,0,0.772043721344145,0.284163499395593,1.24275463521301,2.11073453982987,2.16074473753218,2.3674473558521,1.18058539048442,1.3884040159864,1.39520366850245,1.87791380979733,1.63471479314037,2.63295759861569,0.656669856316381,1.86458261955468,2.92399974850665,1.3173006917463,1.47627060636409,3.15434922846191,2.36680608472354,1.65209391491899,1.85115396664481,0.853153002140936,0.696710015581316,0.902072229254653,0.80673409849337,0.444756087689741,1.14135375667535,0.474529061544512,0.329397909448022,1.00534083520108,0.596869416123452,0.58489226135736,0,1.29682829924817,1.39398891278057,0.569003702859245,1.25673363870702,1.1270773567308,1.22190796707189,1.35766741017338,1.19230893813542,1.39120381673366,1.18088118383682,0.941992367048249,1.35736880313981,3.26097889795864,1.33476084572875,0.982794975566015,0.449724224769707,0.405720242777006,0.72353147925725,0.763877555344259,1.15078454980324,0.972811360650212,1.37280706594041,1.43768206527355,0.372140947668503,2.49630138491648,1.01031551178845,1.07638448113147,1.92977408545347,1.10851914099963,1.70542270593436,1.30371981153621,1.18821560042632,0.469380496577004,1.34005005953453,2.14239276797412,0.975668239288994,1.26108115284953,0.221464689648775,2.87018583583477,0.983179888294428,1.89953480052485,1.44649058581512,0,0,2.45882136509518,0.551873492609465,2.40825884981688,3.5163505977661,1.47541450839008,2.52470927204373,2.2085575359138,1.17131623288992,2.36965737506079,0.477937595905104,2.67358408898873,1.98153571552183,0.809621403349789,2.63030272120181,2.76701444562566,4.81981974632711,2.12910127812403,1.06839362200353,2.11753047159649,1.31976098378028,2.65490276679926,0.728341908464944,0.906970670894253,0.188605079755898,0,2.15204603560749,0.516856586336442,0.727067458955011,1.06949213515416,0.316036757935816,1.05418608574999,1.03135543248187,1.70219802876894,0.900305470156551,1.03486947026204,1.07168517515796,2.30542303734956,1.07021290561242,0.822745103010605,0.608929898229199,2.58138878870081,2.96564899209348,1.54369878638473,1.15420377059036,0.807322133479528,2.17126529741361,1.07435109602823,1.31312719796049,1.7488850197791,3.8939313422207,1.02522528478712,0.412773735926445,1.11116716421827,0.376736914009382,0.546776910884101,0.915112692826091,0.509935289981566,0.979660166265606,0.690999869022119,0,0.790839983952838,0.609958401380546,0.86404272657441,1.04544148007234,1.52853794622324,1.21377911746339,1.32245529160537,1.57053565190418,0.90730481304255,0.621339313086926,27.0572610568411,0.897099276263873,1.48912641411388,2.13896307178452,1.03327858782185,1.85851738039856,1.76035857968631,2.99470085141085,2.9360616439199,9.90494895386625,11.5212002503146,1.89606672376907,0.405450430580328,0.821687657318618,1.16928740680016,1.25318686967237,0.993406773906324,2.28371354087941,1.47689527174577,1.00966449948148,0.838274873204998,1.10908803650525,0.974578807397524,1.53938946624527,1.28564592399726,2.49403421976732,1.99787358036658,2.24284641523689,1.51096154727671,0.965947970160952,1.68914227906064,0.915758121336325,1.50057865355346,1.00314420807073,1.6942555278512,1.84130094602871,3.43737308063638,2.92150409984269,0.685082519770642,3.0215755895588,1.35004500046361,3.60256962579376,2.71885445390523,2.5888015587591,0.830298780673413,1.59609949360412,1.36586884603307,1.45864030240976,1.46729213839588,1.83843508450014,1.55572709461131,1.64828045791115,2.24643446714271,2.02157769411038,2.5347929989024,2.07488975524684,1.68554713445788,1.98477972455095,2.81433797882505,0.476293691283074,1.97003354106254,2.31491064556446,0.824171801606613,1.91358596174717,1.71434626192708,1.77475806159337,2.38436189947321,2.18019624948493,1.69396884747595,2.11536519729191,1.64635175585495,2.63069711437843,2.71608227691724,1.12228454331548,2.01679530811096,1.77308068276014,2.86461730218783,2.94379998253335,1.54325288784913,0.999242311751784,0.467863216890817,1.07435527024149,1.3390183244819,2.20591419119516,3.01428186306548|short_description=p2@Vps37a}} | ||
|EntrezGene=52348 | |||
|HGNC= | |||
|MCL_coexpression_id=0 | |||
|UniProt=Q8CHS8,E9Q7V5,E9Q256,Q3UV99 | |||
|association_with_transcript=0bp_to_ENSMUST00000098817,ENSMUST00000166612,ENSMUST00000172147,NM_033560,uc009lmv.1,uc009lmw.1,uc009lmx.1,uc009lmy.1_5end | |||
|description=CAGE_peak_2_at_Vps37a_5end | |||
|id=chr8:41597123..41597141,+ | |||
|ontology_enrichment_celltype=CL:0000055!4.51e-10!50;CL:0000393!4.24e-09!39;CL:0000211!4.24e-09!39;CL:0000117!1.84e-08!23;CL:0000337!1.84e-08!23;CL:0000221!2.07e-08!44;CL:0000133!2.07e-08!44;CL:0002319!2.13e-08!43;CL:0000540!6.46e-08!33;CL:0000047!6.46e-08!33;CL:0000031!6.46e-08!33;CL:0000404!6.46e-08!33 | |||
|ontology_enrichment_celltype_v019_2=CL:0000393,3.56e-09,39;CL:0000211,3.56e-09,39;CL:0000117,1.66e-08,23;CL:0000337,1.66e-08,23;CL:0000221,1.76e-08,44;CL:0000133,1.76e-08,44;CL:0002319,1.82e-08,43;CL:0000540,5.82e-08,33;CL:0000047,5.82e-08,33;CL:0000031,5.82e-08,33;CL:0000404,5.82e-08,33 | |||
|ontology_enrichment_disease= | |||
|ontology_enrichment_disease_v019_2= | |||
|ontology_enrichment_uberon=UBERON:0000073!3.51e-13!75;UBERON:0001016!3.51e-13!75;UBERON:0001017!3.53e-12!73;UBERON:0005743!3.53e-12!73;UBERON:0004121!1.73e-10!102;UBERON:0000924!1.73e-10!102;UBERON:0006601!1.73e-10!102;UBERON:0001049!1.46e-09!52;UBERON:0005068!1.46e-09!52;UBERON:0006241!1.46e-09!52;UBERON:0007135!1.46e-09!52;UBERON:0002020!7.52e-09!34;UBERON:0000955!1.23e-08!50;UBERON:0006238!1.23e-08!50;UBERON:0002346!1.86e-08!71;UBERON:0003075!1.86e-08!71;UBERON:0007284!1.86e-08!71;UBERON:0002616!3.79e-08!49;UBERON:0000033!7.00e-08!63 | |||
|ontology_enrichment_uberon_v019_2=UBERON:0001016,3.67e-13,75;UBERON:0001017,3.83e-12,73;UBERON:0004121,6.37e-11,95;UBERON:0000924,6.37e-11,95;UBERON:0006601,6.37e-11,95;UBERON:0000073,1.45e-10,54;UBERON:0010314,7.27e-10,92;UBERON:0001049,1.82e-09,52;UBERON:0005068,1.82e-09,52;UBERON:0006241,1.82e-09,52;UBERON:0007135,1.82e-09,52;UBERON:0002346,3.44e-09,64;UBERON:0003075,3.44e-09,64;UBERON:0007284,3.44e-09,64;UBERON:0002020,6.62e-09,34;UBERON:0010371,4.98e-08,73;UBERON:0000955,4.51e-07,47;UBERON:0006238,4.51e-07,47 | |||
|phase1_expression=1.28119077683835,1.04692622291808,0.909998478779072,3.93203474698879,4.50806156951712,1.17515490338323,1.16806320297763,2.7115897922731,0.281577444303333,1.63845168425395,1.71217968225505,1.67521533890206,1.54080656938085,2.27293046289393,0.999329285446988,0.404950534779652,1.39912375625629,0.946386496074713,1.7824876368746,0.84877930359606,7.22867966284093,0.595745707205312,0.783599367416806,0.639969691173627,1.37645568640302,1.14846392823004,1.29228598847722,1.939241094173,0.190023419456374,0.252030129759931,0.665191531965914,0.635030673459631,0,0,0.866554859253824,0.332469290716985,0.408338712524738,2.51118978442145,0,1.57057123192139,4.70585190424811,2.04913645487534,1.45653105909346,1.76255545165808,2.33166941804883,2.20256102594018,1.56478268869608,1.96222640742402,1.31811709640683,1.36100929351998,1.67983705950435,1.43752564364972,0.838913030288682,1.45072428402269,0.336093799142464,1.54355661621568,2.29226751951005,4.12537673821954,2.42779753031517,1.5869729378161,1.90149421051854,2.03306719241479,0,0.899899717281399,0.988866362566974,0.7104801149461,1.19840044207362,2.30530983192933,3.52544681848824,2.70011876663381,3.57552434297349,3.42781181284564,4.19650227808066,1.95985278743926,3.73797336971122,0.919218786610021,4.40463523997555,4.97980618532232,5.9906547969935,1.58263545276233,2.74462930807229,0.52091337055676,5.30447698102624,5.06486349028512,5.04234217663473,5.00868595305235,5.69486723196412,6.15118171223492,4.69502398665741,3.47629176491213,1.15945795480671,3.44169550792555,0,0.539703191637817,0.649776417447669,0.896053774194671,3.37066608316204,1.02258193546835,1.03914401841148,1.7666978725517,1.69012103732017,1.28326477414318,2.54805239706646,0,1.94841936910093,1.01343946190039,1.01104889002957,1.94112595522402,2.33044095282455,3.41416919849097,1.56136794089522,0,0.691846491768175,0.753613202763113,1.76383060703557,2.13633205260448,0.988779870937496,1.01219549843938,0,2.10296904931632,1.76673214768315,0.969750506270444,0,2.04946557908083,2.93225389538837,1.02182822083437,0.239630249011841,0,0.508280908292259,4.6435098215495,1.83000241127451,2.51308704225729,2.43354048269187,3.83573185995496,3.53239675751252,3.56930778749067,1.73126918545538,1.24406970610093,2.52722758433675,2.31486041475121,1.17108398666433,1.37941262660084,1.26043490863744,2.10986744970001,1.42876648217788,0.896796041993948,1.87403449119458,1.81092359517311,0.646703978798527,0.189106080167819,1.05570674498369,1.31517439456545,0.834141747676794,1.39300866608867,0.61734640922383,2.38841120182192,0,0.772043721344145,0.284163499395593,1.24275463521301,2.11073453982987,2.16074473753218,2.3674473558521,1.18058539048442,1.3884040159864,1.39520366850245,1.87791380979733,1.63471479314037,2.63295759861569,0.656669856316381,1.86458261955468,2.92399974850665,1.3173006917463,1.47627060636409,3.15434922846191,2.36680608472354,1.65209391491899,1.85115396664481,0.853153002140936,0.696710015581316,0.902072229254653,0.80673409849337,0.444756087689741,1.14135375667535,0.474529061544512,0.329397909448022,1.00534083520108,0.596869416123452,0.58489226135736,0,1.29682829924817,1.39398891278057,0.569003702859245,1.25673363870702,1.1270773567308,1.22190796707189,1.35766741017338,1.19230893813542,1.39120381673366,1.18088118383682,0.941992367048249,1.35736880313981,3.26097889795864,1.33476084572875,0.982794975566015,0.449724224769707,0.405720242777006,0.72353147925725,0.763877555344259,1.15078454980324,0.972811360650212,1.37280706594041,1.43768206527355,0.372140947668503,2.49630138491648,1.01031551178845,1.07638448113147,1.92977408545347,1.10851914099963,1.70542270593436,1.30371981153621,1.18821560042632,0.469380496577004,1.34005005953453,2.14239276797412,0.975668239288994,1.26108115284953,0.221464689648775,2.87018583583477,0.983179888294428,1.89953480052485,1.44649058581512,0,0,2.45882136509518,0.551873492609465,2.40825884981688,3.5163505977661,1.47541450839008,2.52470927204373,2.2085575359138,1.17131623288992,2.36965737506079,0.477937595905104,2.67358408898873,1.98153571552183,0.809621403349789,2.63030272120181,2.76701444562566,4.81981974632711,2.12910127812403,1.06839362200353,2.11753047159649,1.31976098378028,2.65490276679926,0.728341908464944,0.906970670894253,0.188605079755898,0,2.15204603560749,0.516856586336442,0.727067458955011,1.06949213515416,0.316036757935816,1.05418608574999,1.03135543248187,1.70219802876894,0.900305470156551,1.03486947026204,1.07168517515796,2.30542303734956,1.07021290561242,0.822745103010605,0.608929898229199,2.58138878870081,2.96564899209348,1.54369878638473,1.15420377059036,0.807322133479528,2.17126529741361,1.07435109602823,1.31312719796049,1.7488850197791,3.8939313422207,1.02522528478712,0.412773735926445,1.11116716421827,0.376736914009382,0.546776910884101,0.915112692826091,0.509935289981566,0.979660166265606,0.690999869022119,0,0.790839983952838,0.609958401380546,0.86404272657441,1.04544148007234,1.52853794622324,1.21377911746339,1.32245529160537,1.57053565190418,0.90730481304255,0.621339313086926,27.0572610568411,0.897099276263873,1.48912641411388,2.13896307178452,1.03327858782185,1.85851738039856,1.76035857968631,2.99470085141085,2.9360616439199,9.90494895386625,11.5212002503146,1.89606672376907,0.405450430580328,0.821687657318618,1.16928740680016,1.25318686967237,0.993406773906324,2.28371354087941,1.47689527174577,1.00966449948148,0.838274873204998,1.10908803650525,0.974578807397524,1.53938946624527,1.28564592399726,2.49403421976732,1.99787358036658,2.24284641523689,1.51096154727671,0.965947970160952,1.68914227906064,0.915758121336325,1.50057865355346,1.00314420807073,1.6942555278512,1.84130094602871,3.43737308063638,2.92150409984269,0.685082519770642,3.0215755895588,1.35004500046361,3.60256962579376,2.71885445390523,2.5888015587591,0.830298780673413,1.59609949360412,1.36586884603307,1.45864030240976,1.46729213839588,1.83843508450014,1.55572709461131,1.64828045791115,2.24643446714271,2.02157769411038,2.5347929989024,2.07488975524684,1.68554713445788,1.98477972455095,2.81433797882505,0.476293691283074,1.97003354106254,2.31491064556446,0.824171801606613,1.91358596174717,1.71434626192708,1.77475806159337,2.38436189947321,2.18019624948493,1.69396884747595,2.11536519729191,1.64635175585495,2.63069711437843,2.71608227691724,1.12228454331548,2.01679530811096,1.77308068276014,2.86461730218783,2.94379998253335,1.54325288784913,0.999242311751784,0.467863216890817,1.07435527024149,1.3390183244819,2.20591419119516,3.01428186306548 | |||
|short_description=p2@Vps37a | |||
}} |
Revision as of 15:37, 14 September 2013
Short description: | p2@Vps37a |
---|---|
Species: | Mouse (Mus musculus) |
DPI dataset: | NA |
TSS-like-by-RIKEN-classifier(Yes/No): | NA |
DHS support(Yes/No): | NA |
Description: | CAGE_peak_2_at_Vps37a_5end |
Coexpression cluster: | MCL_coexpression_mm9:0 |
Association with transcript: | 0bp_to_ENSMUST00000098817, ENSMUST00000166612, ENSMUST00000172147, NM_033560, uc009lmv.1, uc009lmw.1, uc009lmx.1, uc009lmy.1_5end |
EntrezGene: | Vps37a |
Link to Zenbu: | ZENBU |
View on UCSC genome browser
CAGE Expression
- Click each plot point to find sample in table
Ontology-based sample term enrichment analysis<b>Summary:</b>This analysis has been performed by utilizing wilcoxon rank sum test.When the number of associated cells/tissues are > n , randomly sampled n cells/tissues are used for P value calculation with rank sum test. this process are repeated several times, and the P values are averaged on the log space <br><b>Analyst:</b> Hideya Kawaji<br><br>link to source dataset<br>data
Ontology term | p-value | n |
---|---|---|
electrically responsive cell | 3.56e-09 | 39 |
electrically active cell | 3.56e-09 | 39 |
CNS neuron (sensu Vertebrata) | 1.66e-08 | 23 |
neuroblast (sensu Vertebrata) | 1.66e-08 | 23 |
ectodermal cell | 1.76e-08 | 44 |
neurectodermal cell | 1.76e-08 | 44 |
neural cell | 1.82e-08 | 43 |
neuron | 5.82e-08 | 33 |
neuronal stem cell | 5.82e-08 | 33 |
neuroblast | 5.82e-08 | 33 |
electrically signaling cell | 5.82e-08 | 33 |
Ontology term | p-value | n |
---|---|---|
nervous system | 3.67e-13 | 75 |
central nervous system | 3.83e-12 | 73 |
ectoderm-derived structure | 6.37e-11 | 95 |
ectoderm | 6.37e-11 | 95 |
presumptive ectoderm | 6.37e-11 | 95 |
regional part of nervous system | 1.45e-10 | 54 |
structure with developmental contribution from neural crest | 7.27e-10 | 92 |
neural tube | 1.82e-09 | 52 |
neural rod | 1.82e-09 | 52 |
future spinal cord | 1.82e-09 | 52 |
neural keel | 1.82e-09 | 52 |
neurectoderm | 3.44e-09 | 64 |
neural plate | 3.44e-09 | 64 |
presumptive neural plate | 3.44e-09 | 64 |
gray matter | 6.62e-09 | 34 |
ecto-epithelium | 4.98e-08 | 73 |
brain | 4.51e-07 | 47 |
future brain | 4.51e-07 | 47 |
Property "Dpi dataset" (as page type) with input value "{{{DPIdataset}}}" contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.
Property "TSS like by RIKEN classifier" (as page type) with input value "{{{TSSclassifier}}}" contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.
Property "DHS support" (as page type) with input value "{{{DHSsupport}}}" contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.