OP-SOLEXA-shortRNA-TruSeq-v2.0

Library constructed with manufacture's protocol [1] with using 'TruSeq Small RNA Sample Prep Kit' (Catalog #: RS-200-0012, RS-200-0024, RS-200-0036, RS-200-0048). This protocol recommend 1-10 μg of total RNA as input, and [RNA_amount] μg of total RNA is used as input. This protocol can use index [2].

References/Notes:

[1] Manufacture's protocol for TruSeq Small RNA Sample Prep Kit.

URL: http://www.illumina.com/products/truseq_small_rna_sample_prep_kit.ilmn

File:TruSeq SmallRNA SamplePrep Guide 15004197 C.pdf

[2] TruSeq Small RNA Sample Prep Index Sequences.

Index Sequence

TruSeq_sRNA_RPI01 ATCACG

TruSeq_sRNA_RPI02 CGATGT

TruSeq_sRNA_RPI03 TTAGGC

TruSeq_sRNA_RPI04 TGACCA

TruSeq_sRNA_RPI05 ACAGTG

TruSeq_sRNA_RPI06 GCCAAT

TruSeq_sRNA_RPI07 CAGATC

TruSeq_sRNA_RPI08 ACTTGA

TruSeq_sRNA_RPI09 GATCAG

TruSeq_sRNA_RPI10 TAGCTT

TruSeq_sRNA_RPI11 GGCTAC

TruSeq_sRNA_RPI12 CTTGTA

TruSeq_sRNA_RPI13 AGTCAA

TruSeq_sRNA_RPI14 AGTTCC

TruSeq_sRNA_RPI15 ATGTCA

TruSeq_sRNA_RPI16 CCGTCC

TruSeq_sRNA_RPI17 GTAGAG

TruSeq_sRNA_RPI18 GTCCGC

TruSeq_sRNA_RPI19 GTGAAA

TruSeq_sRNA_RPI20 GTGGCC

TruSeq_sRNA_RPI21 GTTTCG

TruSeq_sRNA_RPI22 CGTACG

TruSeq_sRNA_RPI23 GAGTGG

TruSeq_sRNA_RPI24 GGTAGC

TruSeq_sRNA_RPI25 CTAGCT

TruSeq_sRNA_RPI26 CTAGCT

TruSeq_sRNA_RPI27 CTATAC

TruSeq_sRNA_RPI28 CTCAGA

TruSeq_sRNA_RPI29 CTGCTG

TruSeq_sRNA_RPI30 TAATCG

TruSeq_sRNA_RPI31 TACAGC

TruSeq_sRNA_RPI32 TATAAT

TruSeq_sRNA_RPI33 TCATTC

TruSeq_sRNA_RPI34 TCCCGA

TruSeq_sRNA_RPI35 TCGAAG

TruSeq_sRNA_RPI36 TCGGCA

TruSeq_sRNA_RPI37 GCCGCG

TruSeq_sRNA_RPI38 GCCTTA

TruSeq_sRNA_RPI39 GCTCCA

TruSeq_sRNA_RPI40 GGCACA

TruSeq_sRNA_RPI41 GGCCTG

TruSeq_sRNA_RPI42 TCTACC

TruSeq_sRNA_RPI43 TGAATG

TruSeq_sRNA_RPI44 TGCCAT

TruSeq_sRNA_RPI45 TGCTGG

TruSeq_sRNA_RPI46 TGGCGC

TruSeq_sRNA_RPI47 TTCGAA

TruSeq_sRNA_RPI48 TTCTCC