OP-SOLEXA-shortRNA-TruSeq-v2.0: Difference between revisions
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[1] Manufacture's protocol for TruSeq Small RNA Sample Prep Kit. | [1] Manufacture's protocol for TruSeq Small RNA Sample Prep Kit. | ||
URL: http://www.illumina.com/products/truseq_small_rna_sample_prep_kit.ilmn | URL: http://www.illumina.com/products/truseq_small_rna_sample_prep_kit.ilmn | ||
File:TruSeq SmallRNA SamplePrep Guide 15004197 C.pdf | File:TruSeq SmallRNA SamplePrep Guide 15004197 C.pdf | ||
Revision as of 11:15, 3 September 2012
Library constructed with manufacture's protocol [1] with using 'TruSeq Small RNA Sample Prep Kit' (Catalog #: RS-200-0012, RS-200-0024, RS-200-0036, RS-200-0048). This protocol recommend 1-10 μg of total RNA as input, and [RNA_amount] μg of total RNA is used as input. This protocol can use index [2].
References/Notes:
[1] Manufacture's protocol for TruSeq Small RNA Sample Prep Kit.
URL: http://www.illumina.com/products/truseq_small_rna_sample_prep_kit.ilmn
File:TruSeq SmallRNA SamplePrep Guide 15004197 C.pdf
[2] TruSeq Small RNA Sample Prep Index Sequences.
Index Sequence
TruSeq_sRNA_RPI01 ATCACG
TruSeq_sRNA_RPI02 CGATGT
TruSeq_sRNA_RPI03 TTAGGC
TruSeq_sRNA_RPI04 TGACCA
TruSeq_sRNA_RPI05 ACAGTG
TruSeq_sRNA_RPI06 GCCAAT
TruSeq_sRNA_RPI07 CAGATC
TruSeq_sRNA_RPI08 ACTTGA
TruSeq_sRNA_RPI09 GATCAG
TruSeq_sRNA_RPI10 TAGCTT
TruSeq_sRNA_RPI11 GGCTAC
TruSeq_sRNA_RPI12 CTTGTA
TruSeq_sRNA_RPI13 AGTCAA
TruSeq_sRNA_RPI14 AGTTCC
TruSeq_sRNA_RPI15 ATGTCA
TruSeq_sRNA_RPI16 CCGTCC
TruSeq_sRNA_RPI17 GTAGAG
TruSeq_sRNA_RPI18 GTCCGC
TruSeq_sRNA_RPI19 GTGAAA
TruSeq_sRNA_RPI20 GTGGCC
TruSeq_sRNA_RPI21 GTTTCG
TruSeq_sRNA_RPI22 CGTACG
TruSeq_sRNA_RPI23 GAGTGG
TruSeq_sRNA_RPI24 GGTAGC
TruSeq_sRNA_RPI25 CTAGCT
TruSeq_sRNA_RPI26 CTAGCT
TruSeq_sRNA_RPI27 CTATAC
TruSeq_sRNA_RPI28 CTCAGA
TruSeq_sRNA_RPI29 CTGCTG
TruSeq_sRNA_RPI30 TAATCG
TruSeq_sRNA_RPI31 TACAGC
TruSeq_sRNA_RPI32 TATAAT
TruSeq_sRNA_RPI33 TCATTC
TruSeq_sRNA_RPI34 TCCCGA
TruSeq_sRNA_RPI35 TCGAAG
TruSeq_sRNA_RPI36 TCGGCA
TruSeq_sRNA_RPI37 GCCGCG
TruSeq_sRNA_RPI38 GCCTTA
TruSeq_sRNA_RPI39 GCTCCA
TruSeq_sRNA_RPI40 GGCACA
TruSeq_sRNA_RPI41 GGCCTG
TruSeq_sRNA_RPI42 TCTACC
TruSeq_sRNA_RPI43 TGAATG
TruSeq_sRNA_RPI44 TGCCAT
TruSeq_sRNA_RPI45 TGCTGG
TruSeq_sRNA_RPI46 TGGCGC
TruSeq_sRNA_RPI47 TTCGAA
TruSeq_sRNA_RPI48 TTCTCC
